Activated iNKT cells promote Vγ9Vδ2-T cell anti-tumor effector functions through the production of TNF-α

Activated iNKT cells promote Vγ9Vδ2-T cell anti-tumor effector functions through the production of TNF-α

Copyright © 2011 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 142(2012), 2 vom: 11. Feb., Seite 194-200
1. Verfasser: Schneiders, Famke L (VerfasserIn)
Weitere Verfasser: de Bruin, Renée C G, Santegoets, Saskia J A M, Bonneville, Marc, Scotet, Emmanuel, Scheper, Rik J, Verheul, Henk M W, de Gruijl, Tanja D, van der Vliet, Hans J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Antigens, Neoplasm Diphosphonates Galactosylceramides Hemiterpenes Immunologic Factors Organophosphorus Compounds Tumor Necrosis Factor-alpha alpha-galactosylceramide mehr... isopentenyl pyrophosphate 358-71-4 Interferon-gamma 82115-62-6
Beschreibung
Zusammenfassung:Copyright © 2011 Elsevier Inc. All rights reserved.
Vγ9Vδ2-T cells constitute a proinflammatory lymphocyte subpopulation with established antitumor activity. Phosphoantigens activate Vγ9Vδ2-T cells in vivo and in vitro. We studied whether the antitumor activity of Vγ9Vδ2-T cells can be potentiated by invariant NKT cells (iNKT), an important immunoregulatory T cell subset. When activated by the glycolipid α-galactosylceramide (α-GalCer), iNKT produce large amounts of cytokines involved in antitumor immune responses. Monocyte-derived dendritic cells were loaded with both phosphoantigens (using aminobisphosphonates) and α-GalCer during maturation and subsequently co-cultured with Vγ9Vδ2-T and iNKT cells. Aminobisphosphonates dose-dependently enhanced Vγ9Vδ2-T cell activation, and this was potentiated by α-GalCer-induced iNKT co-activation. iNKT co-activation also enhanced the IFN-γ production and cytolytic potential of Vγ9Vδ2-T cells against tumor cells. Using transwell experiments and neutralizing antibodies cross-talk between iNKT and Vγ9Vδ2-T cells was found to be mediated by TNF-α. Our data provide a rationale for combining both activating ligands to improve Vγ9Vδ2-T cell based approaches in cancer-immunotherapy
Beschreibung:Date Completed 11.04.2012
Date Revised 06.02.2012
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2011.10.006