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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la202405t
|2 doi
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|a eng
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1 |
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|a Zaccheo, Brian A
|e verfasserin
|4 aut
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|a Stabilization of alkaline phosphatase with AuAg2O nanoparticles
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 13.01.2012
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2011 American Chemical Society
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|a Here, we report that a conductive AuAg(2)O nanoparticle structure significantly enhances the stability of alkaline phosphatase (AlkP) in the presence of the inhibitors urea and l-phenylalanine (Phe). The enzyme/nanoparticle construct is prepared by associating the enzyme with citrate-capped Au particles, and then adding Ag(+). UV-vis and XPS spectroscopy and transmission electron microscopy confirm the core@shell structure. AlkP activity was quantified in the presence and absence of the two inhibitors using a time-resolved colorimetric assay. The results indicate that 21% of the initial active AlkP is incorporated into the nanoparticle structure. More importantly, however, the Au@Ag(2)O core@shell host reduces the inhibitory effect of urea and Phe by factors ranging from 3 to 12, depending on the inhibitor and its concentration, compared to the wild-type enzyme
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Enzyme Inhibitors
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|a Oxides
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|a Silver Compounds
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|a Phenylalanine
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|a 47E5O17Y3R
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|a Urea
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|a Alkaline Phosphatase
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|a EC 3.1.3.1
|2 NLM
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|a Crooks, Richard M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 18 vom: 20. Sept., Seite 11591-6
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:27
|g year:2011
|g number:18
|g day:20
|g month:09
|g pages:11591-6
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|u http://dx.doi.org/10.1021/la202405t
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