Stabilization of alkaline phosphatase with AuAg2O nanoparticles
© 2011 American Chemical Society
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 27(2011), 18 vom: 20. Sept., Seite 11591-6 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2011
|
| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Enzyme Inhibitors Oxides Silver Compounds Phenylalanine 47E5O17Y3R Gold 7440-57-5 mehr... |
| Zusammenfassung: | © 2011 American Chemical Society Here, we report that a conductive AuAg(2)O nanoparticle structure significantly enhances the stability of alkaline phosphatase (AlkP) in the presence of the inhibitors urea and l-phenylalanine (Phe). The enzyme/nanoparticle construct is prepared by associating the enzyme with citrate-capped Au particles, and then adding Ag(+). UV-vis and XPS spectroscopy and transmission electron microscopy confirm the core@shell structure. AlkP activity was quantified in the presence and absence of the two inhibitors using a time-resolved colorimetric assay. The results indicate that 21% of the initial active AlkP is incorporated into the nanoparticle structure. More importantly, however, the Au@Ag(2)O core@shell host reduces the inhibitory effect of urea and Phe by factors ranging from 3 to 12, depending on the inhibitor and its concentration, compared to the wild-type enzyme |
|---|---|
| Beschreibung: | Date Completed 13.01.2012 Date Revised 21.11.2013 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la202405t |