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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1111/j.1939-165X.2011.00286.x
|2 doi
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|a pubmed24n0686.xml
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|a (NLM)21299585
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
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|a Proverbio, Daniela
|e verfasserin
|4 aut
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|a Comparison of gel column agglutination with monoclonal antibodies and card agglutination methods for assessing the feline AB group system and a frequency study of feline blood types in northern Italy
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 07.07.2011
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|a Date Revised 09.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a ©2011 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: A new commercial gel column agglutination system is reported to have high sensitivity in detecting cats with blood type AB
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|a OBJECTIVES: The aims of this study were to compare gel column agglutination and card agglutination methods for feline blood-typing and to determine the frequency distribution of feline blood types in northern Italy
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|a METHODS: Blood-typing was performed on 120 cats using both a commercial gel column containing monoclonal antibodies (ID Gel-Test Micro Typing System) and a card agglutination method (RapidVet-H Feline). Results were confirmed with back-typing. Sensitivity, specificity, positive predictive value, and negative predictive value were calculated for the 2 methods. A second group of 140 Domestic Shorthair (DSH) cats was blood-typed using the gel column technique to determine the frequency distribution of feline blood types in northern Italy
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|a RESULTS: The card agglutination method demonstrated poor sensitivity in identification of type-AB cats (61%) and was only 95% specific when identifying type-B cats. The gel column agglutination technique demonstrated 100% sensitivity and specificity for typing all 3 blood types (A, B, and AB). The frequency distribution study of 140 cats demonstrated that 127 (90.7%) cats were type A, 10 (7.1%) were type B, and 3 (2.1%) were type AB
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|a CONCLUSION: When blood-typing cats of breeds with a relatively high frequency of blood types B and AB, methods that use monoclonal antibodies for detection of blood types B and AB are recommended. Alternatively, blood type can be confirmed by more sensitive supplemental testing, such as back-typing. The high frequency of blood type A in DSH cats in northern Italy was comparable to previously reported frequencies in Italy and world-wide
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|a Comparative Study
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|a Journal Article
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|a Antibodies, Monoclonal
|2 NLM
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| 650 |
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|a Blood Group Antigens
|2 NLM
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| 700 |
1 |
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|a Spada, Eva
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Baggiani, Luciana
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Perego, Roberta
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Milici, Alessandra
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ferro, Elisabetta
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 40(2011), 1 vom: 15. März, Seite 32-9
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnns
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| 773 |
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|g volume:40
|g year:2011
|g number:1
|g day:15
|g month:03
|g pages:32-9
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|u http://dx.doi.org/10.1111/j.1939-165X.2011.00286.x
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