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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erq289
|2 doi
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|a pubmed24n1338.xml
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|a (DE-627)NLM203181123
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|a (NLM)21030387
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Ghosh, Sumit
|e verfasserin
|4 aut
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|a The N-glycan processing enzymes alpha-mannosidase and beta-D-N-acetylhexosaminidase are involved in ripening-associated softening in the non-climacteric fruits of capsicum
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 11.04.2011
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|a Date Revised 21.03.2024
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|a published: Print-Electronic
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|a GENBANK: GU356593, GU356594
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|a Citation Status MEDLINE
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|a Excessive softening of fruits during the ripening process leads to deterioration. This is of significant global importance as softening-mediated deterioration leads to huge postharvest losses. N-glycan processing enzymes are reported to play an important role during climacteric fruit softening: however, to date these enzymes have not been characterized in non-climacteric fruit. Two ripening-specific N-glycan processing enzymes, α-mannosidase (α-Man) and β-D-N-acetylhexosaminidase (β-Hex), have been identified and targeted to enhance the shelf life in non-climacteric fruits such as capsicum (Capsicum annuum). The purification, cloning, and functional characterization of α-Man and β-Hex from capsicum, which belong to glycosyl hydrolase (GH) families 38 and 20, respectively, are described here. α-Man and β-Hex are cell wall glycoproteins that are able to cleave terminal α-mannose and β-D-N-acetylglucosamine residues of N-glycans, respectively. α-Man and β-Hex transcripts as well as enzyme activity increase with the ripening and/or softening of capsicum. The function of α-Man and β-Hex in capsicum softening is investigated through RNA interference (RNAi) in fruits. α-Man and β-Hex RNAi fruits were approximately two times firmer compared with the control and fruit deterioration was delayed by approximately 7 d. It is shown that silencing of α-Man and β-Hex enhances fruit shelf life due to the reduced degradation of N-glycoproteins which resulted in delayed softening. Altogether, the results provide evidence for the involvement of N-glycan processing in non-climacteric fruit softening. In conclusion, genetic engineering of N-glycan processing can be a common strategy in both climacteric and non-climacteric species to reduce the post-harvest crop losses
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Glucans
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Mannosidases
|2 NLM
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|a EC 3.2.1.-
|2 NLM
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|a beta-N-Acetylhexosaminidases
|2 NLM
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| 650 |
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|a EC 3.2.1.52
|2 NLM
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|a Meli, Vijaykumar S
|e verfasserin
|4 aut
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|a Kumar, Anil
|e verfasserin
|4 aut
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|a Thakur, Archana
|e verfasserin
|4 aut
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|a Chakraborty, Niranjan
|e verfasserin
|4 aut
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|a Chakraborty, Subhra
|e verfasserin
|4 aut
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| 700 |
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|a Datta, Asis
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 62(2011), 2 vom: 01. Jan., Seite 571-82
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:62
|g year:2011
|g number:2
|g day:01
|g month:01
|g pages:571-82
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|u http://dx.doi.org/10.1093/jxb/erq289
|3 Volltext
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|d 62
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|h 571-82
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