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231223s2010 xx |||||o 00| ||eng c |
| 024 |
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|a 10.1021/la102489k
|2 doi
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|a pubmed24n0671.xml
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|a (NLM)20809594
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Jung, Hyun Joo
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Selective and direct immobilization of cysteinyl biomolecules by electrochemical cleavage of azo linkage
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| 264 |
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|c 2010
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 03.01.2011
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a Controlled orientation and reserved activity of biomolecules, when site-selectively immobilized in a highly integrated manner on a minimal time scale, are crucial in designing biosensors for the multiplex detection. Here, we describe a novel method for the orientation-controlled immobilization of biomolecules based on site-selective electrochemical activation of p-hydroxyazobenzene self-assembled monolayer (SAM) followed by one-step coupling of cysteinyl biomolecules. The p-aminophenol, a product of reductive cleavage of p-hydroxyazobenzene, was subsequently oxidized to yield p-quinoneimine which then conjugated with cysteinyl biomolecules through 1,4-Michael addition, thus obviating additional linker agents and the related time consumption. Using this method, we selectively activated the electrode surface and immobilized laminin peptide IKVAV, a neurite promoting motif. When we cultured hippocampal neurons on the electrode, the extended neurites were found only within the electrochemically activated area. Hence, the proposed method represents a new promising platform for the patterning of functional peptides, active proteins, and live cells
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Azo Compounds
|2 NLM
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| 650 |
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|a Peptides
|2 NLM
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| 650 |
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|a Cysteine
|2 NLM
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| 650 |
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|a K848JZ4886
|2 NLM
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| 700 |
1 |
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|a Hwang, Inseong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, Beom Jin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Min, Hyegeun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Hyunung
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Tae Geol
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chung, Taek Dong
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 19 vom: 05. Okt., Seite 15087-91
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:26
|g year:2010
|g number:19
|g day:05
|g month:10
|g pages:15087-91
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| 856 |
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|u http://dx.doi.org/10.1021/la102489k
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