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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la102756k
|2 doi
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|a pubmed24n0671.xml
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|a (DE-627)NLM201180790
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|a (NLM)20799750
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Calabretta, Alessandro
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Patterning of peptide nucleic acids using reactive microcontact printing
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| 264 |
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|c 2011
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 26.05.2011
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|a Date Revised 09.02.2011
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive microcontact printing using oxygen-oxidized polydimethylsiloxane stamps, homogeneous arrays were fabricated and characterized using optical methods. PNA-patterned surfaces were hybridized with complementary and mismatched dye-labeled oligonucleotides to test their ability to recognize DNA sequences. The stability and selectivity of the PNA-DNA duplexes on surfaces have been verified by fluorescence microscopy, and the melting curves have been recorded. Finally, the technique has been applied to the fabrication of chips by spotting a PNA microarray onto a flat PDMS stamp and reproducing the same features onto many slides. The chips were finally applied to single nucleotide polymorphism detection on oligonucleotides
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Peptide Nucleic Acids
|2 NLM
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| 700 |
1 |
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|a Wasserberg, Dorothee
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Posthuma-Trumpie, Geertruida A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Subramaniam, Vinod
|e verfasserin
|4 aut
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| 700 |
1 |
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|a van Amerongen, Aart
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Corradini, Roberto
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tedeschi, Tullia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sforza, Stefano
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Reinhoudt, David N
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Marchelli, Rosangela
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huskens, Jurriaan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jonkheijm, Pascal
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 4 vom: 15. Feb., Seite 1536-42
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:27
|g year:2011
|g number:4
|g day:15
|g month:02
|g pages:1536-42
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| 856 |
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