Patterning of peptide nucleic acids using reactive microcontact printing

PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive micro...

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Bibliographische Detailangaben
Veröffentlicht in:	Langmuir : the ACS journal of surfaces and colloids. - 1992. - 27(2011), 4 vom: 15. Feb., Seite 1536-42
1. Verfasser:	Calabretta, Alessandro (VerfasserIn)
Weitere Verfasser:	Wasserberg, Dorothee, Posthuma-Trumpie, Geertruida A, Subramaniam, Vinod, van Amerongen, Aart, Corradini, Roberto, Tedeschi, Tullia, Sforza, Stefano, Reinhoudt, David N, Marchelli, Rosangela, Huskens, Jurriaan, Jonkheijm, Pascal
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2011
Zugriff auf das übergeordnete Werk:	Langmuir : the ACS journal of surfaces and colloids
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Peptide Nucleic Acids

Beschreibung
Zusammenfassung:	PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive microcontact printing using oxygen-oxidized polydimethylsiloxane stamps, homogeneous arrays were fabricated and characterized using optical methods. PNA-patterned surfaces were hybridized with complementary and mismatched dye-labeled oligonucleotides to test their ability to recognize DNA sequences. The stability and selectivity of the PNA-DNA duplexes on surfaces have been verified by fluorescence microscopy, and the melting curves have been recorded. Finally, the technique has been applied to the fabrication of chips by spotting a PNA microarray onto a flat PDMS stamp and reproducing the same features onto many slides. The chips were finally applied to single nucleotide polymorphism detection on oligonucleotides
Beschreibung:	Date Completed 26.05.2011 Date Revised 09.02.2011 published: Print-Electronic Citation Status MEDLINE
ISSN:	1520-5827
DOI:	10.1021/la102756k