|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM200264346 |
| 003 |
DE-627 |
| 005 |
20231223220232.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231223s2010 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/la102456z
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0668.xml
|
| 035 |
|
|
|a (DE-627)NLM200264346
|
| 035 |
|
|
|a (NLM)20704336
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Sebai, Sarra C
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Permeabilization of lipid membranes and cells by a light-responsive copolymer
|
| 264 |
|
1 |
|c 2010
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 27.12.2010
|
| 500 |
|
|
|a Date Revised 10.12.2019
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Membrane permeabilization is achieved via numerous techniques involving the use of molecular agents such as peptides used in antimicrobial therapy. Although high efficiency is reached, the permeabilization mechanism remains global with a noticeable lack of control. To achieve localized control and more gradual increase in membrane perturbation, we have developed hydrophobically modified poly(acrylic acid) amphiphilic copolymers with light-responsive azobenzene hydrophobic moieties. We present evidence for light triggered membrane permeabilization in the presence azobenzene-modified polymers (AMPs). Exposure to UV or blue light reversibly switches the polarity of the azobenzene (cis-trans isomerization) in AMPs, hence controlling AMP-loaded lipid vesicles permeabilization via in situ activation. Release of encapsulated probes was studied by microscopy on isolated AMP-loaded giant unilamellar vesicles (pol-GUVs). We show that in pH and ionic strength conditions that are biologically relevant pol-GUVs are kept impermeable when they contain predominantly cis-AMPs but become leaky with no membrane breakage upon exposure to blue light due to AMPs switch to a trans-apolar state. In addition, we show that AMPs induce destabilization of plasma membranes when added to mammal cells in their trans-apolar state, with no loss of cell viability. These features make AMPs promising tools for remote control of cell membrane permeabilization in mild conditions
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Acrylic Resins
|2 NLM
|
| 650 |
|
7 |
|a Azo Compounds
|2 NLM
|
| 650 |
|
7 |
|a Membrane Lipids
|2 NLM
|
| 650 |
|
7 |
|a carbopol 940
|2 NLM
|
| 650 |
|
7 |
|a 4Q93RCW27E
|2 NLM
|
| 650 |
|
7 |
|a azobenzene
|2 NLM
|
| 650 |
|
7 |
|a F0U1H6UG5C
|2 NLM
|
| 700 |
1 |
|
|a Cribier, Sophie
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Karimi, Ali
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Massotte, Dominique
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Tribet, Christophe
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 17 vom: 07. Sept., Seite 14135-41
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:26
|g year:2010
|g number:17
|g day:07
|g month:09
|g pages:14135-41
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/la102456z
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 26
|j 2010
|e 17
|b 07
|c 09
|h 14135-41
|