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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1021/la901558n
|2 doi
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|a eng
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1 |
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|a Vong, Tuha
|e verfasserin
|4 aut
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|a Site-specific immobilization of DNA in glass microchannels via photolithography
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|c 2009
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|a Text
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|a ƒaComputermedien
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|a Date Completed 22.09.2010
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|a Date Revised 21.06.2010
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|a published: Print
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|a Citation Status MEDLINE
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|a For the first time, a microchannel was photochemically patterned with a functional linker. This simple method was developed for the site-specific attachment of DNA via this linker onto silicon oxide surfaces (e.g., fused silica and borosilicate glass), both onto a flat surface and onto the inside of a fused silica microchannel. Sharp boundaries in the micrometer range between modified and unmodified zones were demonstrated by the attachment of fluorescently labeled DNA oligomers. Studies of repeated hybridization-dehybridization cycles revealed selective and reversible binding of cDNA strands at the explicit locations. On average, approximately 7 x 10(11) fluorescently labeled DNA molecules were hybridized per square centimeter. The modified surfaces were characterized with X-ray photoelectron spectroscopy, infrared microscopy, static contact angle measurements, confocal laser scanning microscopy, and fluorescence detection (to quantify the attachment of the fluorescently labeled DNA)
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fluorescent Dyes
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|a DNA
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|a 9007-49-2
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|a ter Maat, Jurjen
|e verfasserin
|4 aut
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|a van Beek, Teris A
|e verfasserin
|4 aut
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|a van Lagen, Barend
|e verfasserin
|4 aut
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|a Giesbers, Marcel
|e verfasserin
|4 aut
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|a van Hest, Jan C M
|e verfasserin
|4 aut
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|a Zuilhof, Han
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 25(2009), 24 vom: 15. Dez., Seite 13952-8
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|g volume:25
|g year:2009
|g number:24
|g day:15
|g month:12
|g pages:13952-8
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