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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la101192v
|2 doi
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|a pubmed24n0660.xml
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|a (NLM)20450194
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|a DE-627
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Krishnamurthy, Venkata R
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Chemoselective immobilization of peptides on abiotic and cell surfaces at controlled densities
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| 264 |
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 08.09.2010
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|a Date Revised 20.10.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a We report herein a new and enabling approach for decorating both abiotic and cell surfaces with the extracellular matrix IKVAV peptide in a site-specific manner using strain promoted azide-alkyne cycloaddition. A cyclooctyne-derivatized IKVAV peptide was synthesized and immobilized on the surface of pancreatic islets through strain-promoted azide-alkyne cycloaddition with cell surface azides generated by the electrostatic adsorption of a cytocompatible poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) copolymer bearing azido groups (PP-N(3)). Both "one-pot" and sequential addition of PP-N(3) and a cyclooctyne-derivatized IKVAV peptide conjugate enabled efficient modification of the pancreatic islet surface in less than 60 min. The ability to bind peptides at controlled surface densities was demonstrated in a quantitative manner using microarrays. Additionally, the technique is remarkably rapid and highly efficient, opening new avenues for the molecular engineering of cellular interfaces and protein and peptide microarrays
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Peptides
|2 NLM
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| 700 |
1 |
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|a Wilson, John T
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cui, Wanxing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Song, XueZheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lasanajak, Yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cummings, Richard D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chaikof, Elliot L
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 11 vom: 01. Juni, Seite 7675-8
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:26
|g year:2010
|g number:11
|g day:01
|g month:06
|g pages:7675-8
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| 856 |
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|u http://dx.doi.org/10.1021/la101192v
|3 Volltext
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