Thermo-switching of the conformation of genomic DNA in solutions of poly(N-isopropylacrylamide)
We used poly(N-isopropylacrylamide) (PNIPAM) to control the conformation of genomic DNA by changing the temperature of a reaction solution and studied the DNA transition at the level of single DNA molecules. With this method, the conformation of long genomic DNA can be readily and reversibly switche...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1991. - 26(2010), 5 vom: 02. März, Seite 2995-8 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Acrylic Resins Solutions poly-N-isopropylacrylamide 25189-55-3 DNA 9007-49-2 |
| Zusammenfassung: | We used poly(N-isopropylacrylamide) (PNIPAM) to control the conformation of genomic DNA by changing the temperature of a reaction solution and studied the DNA transition at the level of single DNA molecules. With this method, the conformation of long genomic DNA can be readily and reversibly switched between a very compact condensate and an unfolded macromolecule |
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| Beschreibung: | Date Completed 28.05.2010 Date Revised 23.02.2010 published: Print Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la904375k |