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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1111/j.1365-2761.2009.01119.x
|2 doi
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|a pubmed24n0648.xml
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Olivares, J
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Determination of minimal concentration of Piscirickettsia salmonis in water columns to establish a fallowing period in salmon farms
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| 264 |
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|c 2010
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 12.05.2010
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Abstract A highly sensitive real-time PCR procedure to detect and quantify the number of Pisciricketsia salmonis units in seawater samples from affected farm sites has been developed. The purpose was to determine a fallowing period that would allow safe restocking of the target farm with new fish. Bacterial load was determined in water samples by comparing the obtained amplification values against a standard curve generated by the amplification of known concentrations of the ITS-ribosomal component of P. salmonis DNA, cloned in a suitable vector. The standard curve was linear over the range of 10(1)-10(10) log units. Target samples were taken every 10 days over a 40-day period, at 5 m depth and at the surface. In a highly affected area of southern Chile, the number of bacterial units in farm water decreased to zero at day 50. Therefore, a fallowing period of 50 days post-removal of cages of affected fish appears to be appropriate before restocking. This procedure could be adapted to control disease problems because of other pathogens in fish farm waters
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a DNA, Bacterial
|2 NLM
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| 700 |
1 |
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|a Marshall, S H
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 33(2010), 3 vom: 01. März, Seite 261-6
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
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|g volume:33
|g year:2010
|g number:3
|g day:01
|g month:03
|g pages:261-6
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|u http://dx.doi.org/10.1111/j.1365-2761.2009.01119.x
|3 Volltext
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|d 33
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|h 261-6
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