Determination of minimal concentration of Piscirickettsia salmonis in water columns to establish a fallowing period in salmon farms
Abstract A highly sensitive real-time PCR procedure to detect and quantify the number of Pisciricketsia salmonis units in seawater samples from affected farm sites has been developed. The purpose was to determine a fallowing period that would allow safe restocking of the target farm with new fish. B...
| Veröffentlicht in: | Journal of fish diseases. - 1998. - 33(2010), 3 vom: 01. März, Seite 261-6 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | Journal of fish diseases |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't DNA, Bacterial |
| Zusammenfassung: | Abstract A highly sensitive real-time PCR procedure to detect and quantify the number of Pisciricketsia salmonis units in seawater samples from affected farm sites has been developed. The purpose was to determine a fallowing period that would allow safe restocking of the target farm with new fish. Bacterial load was determined in water samples by comparing the obtained amplification values against a standard curve generated by the amplification of known concentrations of the ITS-ribosomal component of P. salmonis DNA, cloned in a suitable vector. The standard curve was linear over the range of 10(1)-10(10) log units. Target samples were taken every 10 days over a 40-day period, at 5 m depth and at the surface. In a highly affected area of southern Chile, the number of bacterial units in farm water decreased to zero at day 50. Therefore, a fallowing period of 50 days post-removal of cages of affected fish appears to be appropriate before restocking. This procedure could be adapted to control disease problems because of other pathogens in fish farm waters |
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| Beschreibung: | Date Completed 12.05.2010 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1365-2761 |
| DOI: | 10.1111/j.1365-2761.2009.01119.x |