Autoimmune T cell responses to antigenic peptides presented by bronchoalveolar lavage cell HLA-DR molecules in sarcoidosis

Autoimmune T cell responses to antigenic peptides presented by bronchoalveolar lavage cell HLA-DR molecules in sarcoidosis

The etiology of sarcoidosis remains unknown. Recently, by mass spectrometric sequencing of peptides eluted from HLA-DR molecules of bronchoalveolar lavage (BAL) cells from DRB10301(pos) patients, we identified potential self-antigens in sarcoidosis. The aim of the present study was to investigate th...

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Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 133(2009), 3 vom: 01. Dez., Seite 353-63
1. Verfasser: Wahlström, Jan (VerfasserIn)
Weitere Verfasser: Dengjel, Jörn, Winqvist, Ola, Targoff, Ira, Persson, Bengt, Duyar, Hüseyin, Rammensee, Hans-Georg, Eklund, Anders, Weissert, Robert, Grunewald, Johan
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Autoantibodies HLA-DR Antigens HLA-DRB1 Chains Peptide Fragments Vimentin DNA 9007-49-2 mehr... Amino Acyl-tRNA Synthetases EC 6.1.1.-
Beschreibung
Zusammenfassung:The etiology of sarcoidosis remains unknown. Recently, by mass spectrometric sequencing of peptides eluted from HLA-DR molecules of bronchoalveolar lavage (BAL) cells from DRB10301(pos) patients, we identified potential self-antigens in sarcoidosis. The aim of the present study was to investigate the capacity of selected peptides to stimulate lung and blood T cells of sarcoidosis patients using an interferon-gamma ELISPOT assay. In peripheral blood, there were strong T cell responses to a peptide derived from the cytoskeletal protein vimentin in 6 out of 11 DRB10301(pos) patients with active disease but not in patients with other HLA types. BAL T cell responses against peptides derived from ATP synthase or from lysyl-tRNA synthetase were detected in DRB10301(pos) as well as DRB10301(neg) patients. By using antigenic peptides presented in vivo in the lungs of sarcoidosis patients, we have identified blood and lung T cell autoimmune responses that may help sustain the inflammation in this disease
Beschreibung:Date Completed 22.12.2009
Date Revised 17.11.2011
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2009.08.008