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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2009.02990.x
|2 doi
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|a eng
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|a Jumpponen, A
|e verfasserin
|4 aut
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|a Massively parallel 454 sequencing indicates hyperdiverse fungal communities in temperate Quercus macrocarpa phyllosphere
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|c 2009
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 03.02.2010
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|a Date Revised 14.04.2021
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|a published: Print-Electronic
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|a CommentIn: New Phytol. 2009 Oct;184(2):279-82. - PMID 19796334
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|a Citation Status MEDLINE
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|a * This study targeted the fungal communities in the phyllosphere of Quercus macrocarpa and compared the fungal species richness, diversity and community composition among trees located within and outside a small urban center using recently developed 454 sequencing and DNA tagging. * The results indicate that the fungal phyllosphere communities are extremely diverse and strongly dominated by ascomycetes, with Microsphaeropsis [two Operational Taxonomic Units (OTUs); 23.6%], Alternaria (six OTUs; 16.1%), Epicoccum (one OTU; 6.0%) and Erysiphe (two OTUs; 5.9%) as the most abundant genera. * Although the sequencing effort averaged 1000 reads per tree and detected nearly 700 distinct molecular OTUs at 95% internal transcribed spacer 1 similarity, the richness of the hyperdiverse phyllosphere communities could not be reliably estimated as nearly one-half of the molecular OTUs were singletons. * The fungal communities within and outside the urban center differed in richness and diversity, which were lower within the urban development. The two land-use types contained communities that were distinct and more than 10% of the molecular OTUs differed in their frequency
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a RNA, Ribosomal
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|a Jones, K L
|e verfasserin
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|i Enthalten in
|t The New phytologist
|d 1979
|g 184(2009), 2 vom: 01. Okt., Seite 438-448
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|x 1469-8137
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|g pages:438-448
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