Massively parallel 454 sequencing indicates hyperdiverse fungal communities in temperate Quercus macrocarpa phyllosphere

* This study targeted the fungal communities in the phyllosphere of Quercus macrocarpa and compared the fungal species richness, diversity and community composition among trees located within and outside a small urban center using recently developed 454 sequencing and DNA tagging. * The results indi...

Description complète

Détails bibliographiques
Publié dans:The New phytologist. - 1990. - 184(2009), 2 vom: 01. Okt., Seite 438-448
Auteur principal: Jumpponen, A (Auteur)
Autres auteurs: Jones, K L
Format: Article en ligne
Langue:English
Publié: 2009
Accès à la collection:The New phytologist
Sujets:Journal Article Research Support, Non-U.S. Gov't RNA, Ribosomal
Description
Résumé:* This study targeted the fungal communities in the phyllosphere of Quercus macrocarpa and compared the fungal species richness, diversity and community composition among trees located within and outside a small urban center using recently developed 454 sequencing and DNA tagging. * The results indicate that the fungal phyllosphere communities are extremely diverse and strongly dominated by ascomycetes, with Microsphaeropsis [two Operational Taxonomic Units (OTUs); 23.6%], Alternaria (six OTUs; 16.1%), Epicoccum (one OTU; 6.0%) and Erysiphe (two OTUs; 5.9%) as the most abundant genera. * Although the sequencing effort averaged 1000 reads per tree and detected nearly 700 distinct molecular OTUs at 95% internal transcribed spacer 1 similarity, the richness of the hyperdiverse phyllosphere communities could not be reliably estimated as nearly one-half of the molecular OTUs were singletons. * The fungal communities within and outside the urban center differed in richness and diversity, which were lower within the urban development. The two land-use types contained communities that were distinct and more than 10% of the molecular OTUs differed in their frequency
Description:Date Completed 03.02.2010
Date Revised 09.01.2024
published: Print-Electronic
CommentIn: New Phytol. 2009 Oct;184(2):279-282. doi: 10.1111/j.1469-8137.2009.03042.x. - PMID 19796334
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/j.1469-8137.2009.02990.x