Metabonomic investigations into the global biochemical sequelae of exposure to the pancreatic toxin 1-cyano-2-hydroxy-3-butene in the rat

The time-related metabolic effects of 1-cyano-2-hydroxy-3-butene (CHB, crambene), a naturally occurring nitrile and experimental model toxin causing exocrine pancreatitis, have been investigated in rats using high-resolution NMR spectroscopy of urine and serum in combination with pattern recognition...

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Veröffentlicht in:	Magnetic resonance in chemistry : MRC. - 1985. - 47 Suppl 1(2009) vom: 01. Dez., Seite S26-35
1. Verfasser:	Bohus, Eszter (VerfasserIn)
Weitere Verfasser:	Rácz, Akos, Noszál, Béla, Coen, Muireann, Beckonert, Olaf, Keun, Hector C, Ebbels, Timothy M D, Cantor, Glenn H, Wijsman, John A, Holmes, Elaine, Lindon, John C, Nicholson, Jeremy K
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2009
Zugriff auf das übergeordnete Werk:	Magnetic resonance in chemistry : MRC
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Alkenes Nitriles 1-cyano-2-hydroxy-3-butene 27451-36-1

Beschreibung
Zusammenfassung:	The time-related metabolic effects of 1-cyano-2-hydroxy-3-butene (CHB, crambene), a naturally occurring nitrile and experimental model toxin causing exocrine pancreatitis, have been investigated in rats using high-resolution NMR spectroscopy of urine and serum in combination with pattern recognition analysis. Rats were administered CHB subcutaneously in two doses, 15 mg/kg dose (n = 10) and 150 mg/kg (n = 10), and conventional histopathology and clinical chemistry assessments were performed. Urine samples were collected at - 16 and 0, 8, 24, 48, 72, 96, 120, 144 and 168 h postdosing and serum samples were collected at 48 and 168 h postdosing; these were analyzed using a range of 1D and 2D NMR spectroscopic methods. The metabolic profile perturbations seen throughout the time-course of the study are described, and the application of the spectral correlation technique Statistical TOtal Correlation SpectroscopY (STOCSY) to detect both structural and novel toxicological connectivities between xenobiotic and endogenous metabolite signals is illustrated for the first time. As a result, it is suggested that the STOCSY approach may be of wider application in the identification of toxic versus nontoxic metabolites in drug metabolism studies
Beschreibung:	Date Completed 21.04.2010 Date Revised 12.11.2009 published: Print Citation Status MEDLINE
ISSN:	1097-458X
DOI:	10.1002/mrc.2485