T cell CD40LG gene expression and the production of IgG by autologous B cells in systemic lupus erythematosus

T cell CD40LG gene expression and the production of IgG by autologous B cells in systemic lupus erythematosus

CD40 ligand (CD40LG), encoded on the X chromosome, has been reported to be overexpressed on lupus T cells. Herein, we investigated the effect of DNA demethylation on T cell CD40LG expression and the production of IgG by autologous B cells in lupus. We found normal human T cells transfected with CD40...

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Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 132(2009), 3 vom: 15. Sept., Seite 362-70
1. Verfasser: Zhou, Ying (VerfasserIn)
Weitere Verfasser: Yuan, Jun, Pan, Yujun, Fei, Yiping, Qiu, Xiangning, Hu, Nan, Luo, Yongqi, Lei, Wenzhi, Li, Yaping, Long, Hai, Sawalha, Amr H, Richardson, Bruce, Lu, Qianjin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Antibodies, Monoclonal Antigens, CD Enzyme Inhibitors Immunoglobulin G Lipopolysaccharides CD40 Ligand 147205-72-9
Beschreibung
Zusammenfassung:CD40 ligand (CD40LG), encoded on the X chromosome, has been reported to be overexpressed on lupus T cells. Herein, we investigated the effect of DNA demethylation on T cell CD40LG expression and the production of IgG by autologous B cells in lupus. We found normal human T cells transfected with CD40LG induced autologous B cell activation and plasma cell differentiation. Both female lupus CD4+ T cells and demethylating agents treated CD4+ T cells overexpressed CD40LG mRNA. Further, lupus T cells from both genders or demethylated CD4+ T cells from healthy women overstimulated autologous B cells, and this could be reversed with anti-CD40LG Ab in only females. We demonstrated that female lupus CD4+ T cells and demethylated CD4+ T cells express high level of CD40LG and overstimulate B cells to produce IgG. This is due to DNA demethylation and thereby reactivation of the inactive X chromosome in female
Beschreibung:Date Completed 01.09.2009
Date Revised 20.10.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2009.05.011