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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1002/mrc.2444
|2 doi
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|a pubmed24n0629.xml
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|a (DE-627)NLM188550917
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|a (NLM)19444862
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Chu, Shidong
|e verfasserin
|4 aut
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|a Solid-state NMR spectroscopic studies on the interaction of sorbic acid with phospholipid membranes at different pH levels
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 16.09.2009
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|a Date Revised 20.10.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a 2H, 31P, and 1H-magic-angle-spinning (MAS) solid-state NMR spectroscopic methods were used to elucidate the interaction between sorbic acid, a widely used weak acid food preservative, and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bilayers under both acidic and neutral pH conditions. The linewidth broadening observed in the 31P NMR powder pattern spectra and the changes in the 31P longitudinal relaxation time (T1) indicate interaction with the phospholipid headgroup upon titration of sorbic acid or decanoic acid into DMPC bilayers over the pH range from 3.0 to 7.4. The peak intensities of sorbic acid decrease upon addition of paramagnetic Mn2+ ions in DMPC bilayers as recorded in the 1H MAS NMR spectra, suggesting that sorbic acid molecules are in close proximity with the membrane/aqueous surface. No significant 2H quadrupolar splitting (DeltanuQ) changes are observed in the 2H NMR spectra of DMPC-d54 upon titration of sorbic acid, and the change of pH has a slight effect on DeltanuQ, indicating that sorbic acid has weak influence on the orientation order of the DMPC acyl chains in the fluid phase over the pH range from 3.0 to 7.4. This finding is in contrast to the results of the decanoic acid/DMPC-d54 systems, where DeltanuQ increases as the concentration of decanoic acid increases. Thus, in the membrane association process, sorbic acids are most likely interacting with the headgroups and shallowly embedded near the top of the phospholipid headgroups, rather than inserting deep into the acyl chains. Thus, antimicrobial mode of action for sorbic acid may be different from that of long-chain fatty acids
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Food Preservatives
|2 NLM
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|a Lipid Bilayers
|2 NLM
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|a Phospholipids
|2 NLM
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|a Dimyristoylphosphatidylcholine
|2 NLM
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| 650 |
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|a U86ZGC74V5
|2 NLM
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| 650 |
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|a Sorbic Acid
|2 NLM
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| 650 |
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|a X045WJ989B
|2 NLM
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| 700 |
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|a Hawes, John W
|e verfasserin
|4 aut
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| 700 |
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|a Lorigan, Gary A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Magnetic resonance in chemistry : MRC
|d 1985
|g 47(2009), 8 vom: 01. Aug., Seite 651-7
|w (DE-627)NLM098179667
|x 1097-458X
|7 nnns
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| 773 |
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|g volume:47
|g year:2009
|g number:8
|g day:01
|g month:08
|g pages:651-7
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|u http://dx.doi.org/10.1002/mrc.2444
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