Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells

Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting o...

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Détails bibliographiques
Publié dans:BioTechniques. - 1993. - 46(2009), 1 vom: 21. Jan., Seite 21-2, 24, 26 passim
Auteur principal: Matentzoglu, Konstantin (Auteur)
Autres auteurs: Scheffner, Martin
Format: Article
Langue:English
Publié: 2009
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't Technical Report Recombinant Fusion Proteins Ubiquitin enhanced green fluorescent protein Green Fluorescent Proteins 147336-22-9 UBE3A protein, human EC 2.3.2.26 plus... Ubiquitin-Protein Ligases EC 2.3.2.27