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20250210064902.0 |
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tu |
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231223s2009 xx ||||| 00| ||eng c |
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|a pubmed25n0624.xml
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|a (NLM)19301619
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Matentzoglu, Konstantin
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Ubiquitin-fusion protein system
|b a powerful tool for ectopic protein expression in mammalian cells
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| 264 |
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1 |
|c 2009
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 30.04.2009
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|a Date Revised 07.06.2018
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest
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| 650 |
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|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Technical Report
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| 650 |
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7 |
|a Recombinant Fusion Proteins
|2 NLM
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| 650 |
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7 |
|a Ubiquitin
|2 NLM
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| 650 |
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7 |
|a enhanced green fluorescent protein
|2 NLM
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| 650 |
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7 |
|a Green Fluorescent Proteins
|2 NLM
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| 650 |
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7 |
|a 147336-22-9
|2 NLM
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| 650 |
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7 |
|a UBE3A protein, human
|2 NLM
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| 650 |
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7 |
|a EC 2.3.2.26
|2 NLM
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| 650 |
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7 |
|a Ubiquitin-Protein Ligases
|2 NLM
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| 650 |
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7 |
|a EC 2.3.2.27
|2 NLM
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| 700 |
1 |
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|a Scheffner, Martin
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 46(2009), 1 vom: 21. Jan., Seite 21-2, 24, 26 passim
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:46
|g year:2009
|g number:1
|g day:21
|g month:01
|g pages:21-2, 24, 26 passim
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|d 46
|j 2009
|e 1
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|h 21-2, 24, 26 passim
|