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01000caa a22002652c 4500 |
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NLM186998872 |
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DE-627 |
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20250210061519.0 |
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tu |
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231223s1995 xx ||||| 00| ||eng c |
| 028 |
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2 |
|a pubmed25n0623.xml
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|a (DE-627)NLM186998872
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| 035 |
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|a (NLM)19277286
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Stamps, W T
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A rapid and simple method for staining lipid in fixed nematodes
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| 264 |
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1 |
|c 1995
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 14.07.2011
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| 500 |
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|a Date Revised 20.10.2021
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a A method is described for staining lipid in fourth-stage dispersal juvenile nematodes fixed with formal-acetic fixative (FA4:1). Bursaphelenchus xylophilus fourth-stage dispersal juveniles were fixed with hot FA4:1 for 24 hours, excess fixative was removed, and a solution of saturated oil red O in 96% ethanol added and allowed to sit for 25 minutes at 60 C. Excess oil red O was removed, nematodes were washed twice with 70% ethanol, and were processed to pure glycerin. Lipid droplets within the nematodes were viewed by light microscopy and appeared as dark red spheres of various sizes. Computerized image analysis was used to quantify lipid droplet area
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Bursaphelenchm xylophilus
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| 650 |
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4 |
|a lipid
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| 650 |
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4 |
|a oil red O
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| 650 |
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4 |
|a pine wilt
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| 650 |
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4 |
|a pinewood nematode
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| 650 |
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4 |
|a staining
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| 700 |
1 |
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|a Linit, M J
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of nematology
|d 1969
|g 27(1995), 2 vom: 01. Juni, Seite 244-8
|w (DE-627)NLM098196421
|x 0022-300X
|7 nnas
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| 773 |
1 |
8 |
|g volume:27
|g year:1995
|g number:2
|g day:01
|g month:06
|g pages:244-8
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 27
|j 1995
|e 2
|b 01
|c 06
|h 244-8
|