A rapid and simple method for staining lipid in fixed nematodes
A method is described for staining lipid in fourth-stage dispersal juvenile nematodes fixed with formal-acetic fixative (FA4:1). Bursaphelenchus xylophilus fourth-stage dispersal juveniles were fixed with hot FA4:1 for 24 hours, excess fixative was removed, and a solution of saturated oil red O in 9...
| Publié dans: | Journal of nematology. - 1969. - 27(1995), 2 vom: 01. Juni, Seite 244-8 |
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| Format: | Article |
| Langue: | English |
| Publié: |
1995
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| Accès à la collection: | Journal of nematology |
| Sujets: | Journal Article Bursaphelenchm xylophilus lipid oil red O pine wilt pinewood nematode staining |
| Résumé: | A method is described for staining lipid in fourth-stage dispersal juvenile nematodes fixed with formal-acetic fixative (FA4:1). Bursaphelenchus xylophilus fourth-stage dispersal juveniles were fixed with hot FA4:1 for 24 hours, excess fixative was removed, and a solution of saturated oil red O in 96% ethanol added and allowed to sit for 25 minutes at 60 C. Excess oil red O was removed, nematodes were washed twice with 70% ethanol, and were processed to pure glycerin. Lipid droplets within the nematodes were viewed by light microscopy and appeared as dark red spheres of various sizes. Computerized image analysis was used to quantify lipid droplet area |
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| Description: | Date Completed 14.07.2011 Date Revised 20.10.2021 published: Print Citation Status PubMed-not-MEDLINE |
| ISSN: | 0022-300X |