Atomic force microscopy of plant-parasitic nematodes

A simple method for atomic force microscopy (AFM) of nematode cuticle was developed to visualize the external topography of Helicotylenchus lobus, Meloidogyne javanica, M. incognita, and Xiphinema diversicaudatum. Endospores of two isolates of the nematode parasite, Pasteuria penetrans, adhering to...

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Veröffentlicht in:Journal of nematology. - 1969. - 27(1995), 2 vom: 01. Juni, Seite 200-5
1. Verfasser: Ciancio, A (VerfasserIn)
Format: Aufsatz
Sprache:English
Veröffentlicht: 1995
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article AFM Pasteuria penetrans atomic force microscope cuticle endospore morphology nematode scanning probe microscopy ultrastructure
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245 1 0 |a Atomic force microscopy of plant-parasitic nematodes 
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520 |a A simple method for atomic force microscopy (AFM) of nematode cuticle was developed to visualize the external topography of Helicotylenchus lobus, Meloidogyne javanica, M. incognita, and Xiphinema diversicaudatum. Endospores of two isolates of the nematode parasite, Pasteuria penetrans, adhering to M. incognita and X. diversicaudatum were also visualized and measured by this technique. Scanning procedures were applied to specimens killed and dehydrated in air or dehydrated and stored in glycerol. Atomic force microscopy scanning of nematodes in constant height mode yielded replicated high-resolution images of the cuticle showing anatomical details such as annulations and lateral fields. Submicrometer scale images allowed the identification of planar regions for further higher resolution scans 
650 4 |a Journal Article 
650 4 |a AFM 
650 4 |a Pasteuria penetrans 
650 4 |a atomic force microscope 
650 4 |a cuticle 
650 4 |a endospore 
650 4 |a morphology 
650 4 |a nematode 
650 4 |a scanning probe microscopy 
650 4 |a ultrastructure 
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