A Comparative Analysis of Extraction Methods for the Recovery of Anguina sp. from Grass Seed Samples

Four procedures were compared in their efficacy to extract juveniles of Anguina agrostis from commercial grass seed. The procedures included those currently used by the state regulatory laboratories of Oregon and California, as well as new tests developed to determine juvenile viability for the phyt...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Journal of nematology. - 1969. - 31(1999), 4S vom: 16. Dez., Seite 635-40
1. Verfasser: Griesbach, J A (VerfasserIn)
Weitere Verfasser: Chitambar, J J, Hamerlynck, M J, Duarte, E O
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Anguina agrostis assay bentgrass extraction procedures nematode orchardgrass phytosanitary certification regulatory nematology seed-borne nematodes technique
LEADER 01000caa a22002652 4500
001 NLM186939205
003 DE-627
005 20250210060450.0
007 tu
008 231223s1999 xx ||||| 00| ||eng c
028 5 2 |a pubmed25n0623.xml 
035 |a (DE-627)NLM186939205 
035 |a (NLM)19270928 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Griesbach, J A  |e verfasserin  |4 aut 
245 1 2 |a A Comparative Analysis of Extraction Methods for the Recovery of Anguina sp. from Grass Seed Samples 
264 1 |c 1999 
336 |a Text  |b txt  |2 rdacontent 
337 |a ohne Hilfsmittel zu benutzen  |b n  |2 rdamedia 
338 |a Band  |b nc  |2 rdacarrier 
500 |a Date Completed 14.07.2011 
500 |a Date Revised 20.10.2021 
500 |a published: Print 
500 |a Citation Status PubMed-not-MEDLINE 
520 |a Four procedures were compared in their efficacy to extract juveniles of Anguina agrostis from commercial grass seed. The procedures included those currently used by the state regulatory laboratories of Oregon and California, as well as new tests developed to determine juvenile viability for the phytosanitary certification of fumigated grass seed. Eleven seed lots of Agrostis tenuis (bentgrass) and Dactylis glomerata (orchardgrass) naturally infested with varying levels of juveniles of Anguina were individually analyzed. Only one procedure, a new live recovery test, yielded nematodes in all 11 samples and is recommended as the best method for use by regulatory agencies. In comparison, although the other three extraction procedures resulted in greater numbers of Anguina agrostis juveniles per gram of seed, they failed to yield any nematodes in as many as four seed lots with low infection levels 
650 4 |a Journal Article 
650 4 |a Anguina agrostis 
650 4 |a assay 
650 4 |a bentgrass 
650 4 |a extraction procedures 
650 4 |a nematode 
650 4 |a orchardgrass 
650 4 |a phytosanitary certification 
650 4 |a regulatory nematology 
650 4 |a seed-borne nematodes 
650 4 |a technique 
700 1 |a Chitambar, J J  |e verfasserin  |4 aut 
700 1 |a Hamerlynck, M J  |e verfasserin  |4 aut 
700 1 |a Duarte, E O  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of nematology  |d 1969  |g 31(1999), 4S vom: 16. Dez., Seite 635-40  |w (DE-627)NLM098196421  |x 0022-300X  |7 nnns 
773 1 8 |g volume:31  |g year:1999  |g number:4S  |g day:16  |g month:12  |g pages:635-40 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 31  |j 1999  |e 4S  |b 16  |c 12  |h 635-40