A Comparative Analysis of Extraction Methods for the Recovery of Anguina sp. from Grass Seed Samples
Four procedures were compared in their efficacy to extract juveniles of Anguina agrostis from commercial grass seed. The procedures included those currently used by the state regulatory laboratories of Oregon and California, as well as new tests developed to determine juvenile viability for the phyt...
| Veröffentlicht in: | Journal of nematology. - 1969. - 31(1999), 4S vom: 16. Dez., Seite 635-40 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1999
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| Zugriff auf das übergeordnete Werk: | Journal of nematology |
| Schlagworte: | Journal Article Anguina agrostis assay bentgrass extraction procedures nematode orchardgrass phytosanitary certification regulatory nematology seed-borne nematodes |
| Zusammenfassung: | Four procedures were compared in their efficacy to extract juveniles of Anguina agrostis from commercial grass seed. The procedures included those currently used by the state regulatory laboratories of Oregon and California, as well as new tests developed to determine juvenile viability for the phytosanitary certification of fumigated grass seed. Eleven seed lots of Agrostis tenuis (bentgrass) and Dactylis glomerata (orchardgrass) naturally infested with varying levels of juveniles of Anguina were individually analyzed. Only one procedure, a new live recovery test, yielded nematodes in all 11 samples and is recommended as the best method for use by regulatory agencies. In comparison, although the other three extraction procedures resulted in greater numbers of Anguina agrostis juveniles per gram of seed, they failed to yield any nematodes in as many as four seed lots with low infection levels |
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| Beschreibung: | Date Completed 14.07.2011 Date Revised 20.10.2021 published: Print Citation Status PubMed-not-MEDLINE |
| ISSN: | 0022-300X |