Detection and Partial Characterization of Egg Polypeptides from Heterodera glycines

The presence of two major egg polypeptides was demonstrated in the plant-parasitic nematode Heterodera glycines. The polypeptides were present in equal amounts in, and were most abundant in, eggs from yellow females. They were also present in brown females but were not detected in second-stage juven...

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Veröffentlicht in:Journal of nematology. - 1969. - 31(1999), 3 vom: 16. Sept., Seite 305-11
1. Verfasser: Masler, E P (VerfasserIn)
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Caenorhabditis elegans Dolichorhabditis sp. Heterodera glycines egg electrophoresis female development major egg polypeptide nematode protein mehr... reproductive physiology soybean cyst nematode vitellin vitellogenin
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245 1 0 |a Detection and Partial Characterization of Egg Polypeptides from Heterodera glycines 
264 1 |c 1999 
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500 |a Date Completed 14.07.2011 
500 |a Date Revised 20.10.2021 
500 |a published: Print 
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520 |a The presence of two major egg polypeptides was demonstrated in the plant-parasitic nematode Heterodera glycines. The polypeptides were present in equal amounts in, and were most abundant in, eggs from yellow females. They were also present in brown females but were not detected in second-stage juveniles (J2). The two major egg polypeptides, MEP-I and MEP-II, accounted for more than 50% of the total protein in egg extracts evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. During development of females from the yellow stage to the brown stage, the levels of MEP-I and MEP-II declined at twice the rate as total protein. MEP-I and MEP-II had estimated molecular masses of 190 kD and 180 kD, respectively, similar to those reported for female-specific proteins, vitellins, from free-living nematodes 
650 4 |a Journal Article 
650 4 |a Caenorhabditis elegans 
650 4 |a Dolichorhabditis sp. 
650 4 |a Heterodera glycines 
650 4 |a egg 
650 4 |a electrophoresis 
650 4 |a female development 
650 4 |a major egg polypeptide 
650 4 |a nematode 
650 4 |a protein 
650 4 |a reproductive physiology 
650 4 |a soybean cyst nematode 
650 4 |a vitellin 
650 4 |a vitellogenin 
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