Detection and Partial Characterization of Egg Polypeptides from Heterodera glycines
The presence of two major egg polypeptides was demonstrated in the plant-parasitic nematode Heterodera glycines. The polypeptides were present in equal amounts in, and were most abundant in, eggs from yellow females. They were also present in brown females but were not detected in second-stage juven...
Veröffentlicht in: | Journal of nematology. - 1969. - 31(1999), 3 vom: 16. Sept., Seite 305-11 |
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1. Verfasser: | |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
1999
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Zugriff auf das übergeordnete Werk: | Journal of nematology |
Schlagworte: | Journal Article Caenorhabditis elegans Dolichorhabditis sp. Heterodera glycines egg electrophoresis female development major egg polypeptide nematode protein mehr... |
Zusammenfassung: | The presence of two major egg polypeptides was demonstrated in the plant-parasitic nematode Heterodera glycines. The polypeptides were present in equal amounts in, and were most abundant in, eggs from yellow females. They were also present in brown females but were not detected in second-stage juveniles (J2). The two major egg polypeptides, MEP-I and MEP-II, accounted for more than 50% of the total protein in egg extracts evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. During development of females from the yellow stage to the brown stage, the levels of MEP-I and MEP-II declined at twice the rate as total protein. MEP-I and MEP-II had estimated molecular masses of 190 kD and 180 kD, respectively, similar to those reported for female-specific proteins, vitellins, from free-living nematodes |
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Beschreibung: | Date Completed 14.07.2011 Date Revised 20.10.2021 published: Print Citation Status PubMed-not-MEDLINE |
ISSN: | 0022-300X |