Layer-by-layer technique as a new approach to produce nanostructured films containing phospholipids as transducers in sensing applications
Phospholipids are widely used as mimetic systems to exploit interactions involving biological membranes and pharmacological drugs. In this work, the layer-by-layer (LbL) technique was used as a new approach to produce multilayered thin films containing biological phospholipids applied as transducers...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 25(2009), 4 vom: 17. Feb., Seite 2331-8 |
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| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2009
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Pharmaceutical Preparations Phospholipids |
| Zusammenfassung: | Phospholipids are widely used as mimetic systems to exploit interactions involving biological membranes and pharmacological drugs. In this work, the layer-by-layer (LbL) technique was used as a new approach to produce multilayered thin films containing biological phospholipids applied as transducers onto Pt interdigitated electrodes forming sensing units of an electronic tongue system. Low concentrations (nM level) of a phenothiazine compound were detected through impedance spectroscopy. Both negative 1,2-dipalmitoyl-sn-3-glycero-[phosphor-rac-(1-glycerol)] (DPPG) and zwitterionic l-alpha-1,2-dipalmitoyl-sn-3-glycero-phosphatidylcholine (DPPC) phospholipids were used to produce the LbL films, whose molecular architecture was monitored combining spectroscopy and microscopy at micro and nanoscales. The sensor array was complemented by Langmuir-Blodgett (LB) monolayers of DPPG and DPPC deposited onto Pt interdigitated electrodes as well. It was found that the distinct molecular architecture presented by both LbL and LB films plays a key role on the sensitivity of the sensor array with the importance of the LbL films being demonstrated by principal component analysis (PCA) |
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| Beschreibung: | Date Completed 31.03.2009 Date Revised 09.03.2009 published: Print Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la802696j |