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231223s2009 xx |||||o 00| ||eng c |
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7 |
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|a 10.1021/la803597m
|2 doi
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|a eng
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| 100 |
1 |
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|a Heyries, Kevin A
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Straightforward protein immobilization using redox-initiated poly(methyl methacrylate) polymerization
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 27.02.2009
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|a Date Revised 17.11.2011
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|a published: Print
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|a Citation Status MEDLINE
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|a Rigid poly(methyl methacrylate) (PMMA) biochips directly modified with active protein spots were obtained, using a redox-initiated PMMA polymerization process. The protein immobilization mechanism is believed to be a combination of both a covalent binding through transient amino acid radical generation and a direct entrapment of the biomolecules in the PMMA polymer. Three different immunoassays (binding, capture, and sandwich) were performed using the developed system, and really promising limits of detection (160-200pg/mL) were obtained, demonstrating a novel straightforward route to fabricate plastic biochips
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|a Journal Article
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|a Immobilized Proteins
|2 NLM
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|a Polymethyl Methacrylate
|2 NLM
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|a 9011-14-7
|2 NLM
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| 700 |
1 |
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|a Blum, Loïc J
|e verfasserin
|4 aut
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1 |
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|a Marquette, Christophe A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 25(2009), 2 vom: 20. Jan., Seite 661-4
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