Straightforward protein immobilization using redox-initiated poly(methyl methacrylate) polymerization
Rigid poly(methyl methacrylate) (PMMA) biochips directly modified with active protein spots were obtained, using a redox-initiated PMMA polymerization process. The protein immobilization mechanism is believed to be a combination of both a covalent binding through transient amino acid radical generat...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 25(2009), 2 vom: 20. Jan., Seite 661-4 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2009
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Immobilized Proteins Polymethyl Methacrylate 9011-14-7 |
| Zusammenfassung: | Rigid poly(methyl methacrylate) (PMMA) biochips directly modified with active protein spots were obtained, using a redox-initiated PMMA polymerization process. The protein immobilization mechanism is believed to be a combination of both a covalent binding through transient amino acid radical generation and a direct entrapment of the biomolecules in the PMMA polymer. Three different immunoassays (binding, capture, and sandwich) were performed using the developed system, and really promising limits of detection (160-200pg/mL) were obtained, demonstrating a novel straightforward route to fabricate plastic biochips |
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| Beschreibung: | Date Completed 27.02.2009 Date Revised 17.11.2011 published: Print Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la803597m |