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01000caa a22002652 4500 |
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NLM183723805 |
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DE-627 |
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20250209203606.0 |
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tu |
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231223s2008 xx ||||| 00| ||chi c |
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|a pubmed25n0612.xml
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|a (DE-627)NLM183723805
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|a (NLM)18926065
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Wang, Nan
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Effect of endotoxin on expression of angiotensin converting enzyme-2 in cultured rat pulmonary microvascular endothelial cells
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| 264 |
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|c 2008
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 09.03.2010
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|a Date Revised 09.12.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To observe the effects of endotoxin on angiotensin converting enzyme-2 (ACE2) expression in rat pulmonary microvascular endothelial cells (RPMVECs) in vitro, and discuss the role of ACE2 in endotoxin-induced acute lung injury (ALI)
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|a METHODS: In vitro, cultured RPMVECs were incubated with endotoxin (10 mg/L), the expression of ACE2 mRNA and protein were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting techniques at time points of 3, 6, 12, and 24 hours respectively after incubation with endotoxin
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|a RESULTS: After the treatment of RPMVECs with endotoxin (10 mg/L), the levels of ACE2 mRNA and protein decreased significantly, and then they increased slightly at 6 hours, followed by a continuous decrease until 24 hours. The statistic analysis demonstrated that, except at the 6-hour time point, the levels of ACE2 mRNA and protein expression at other time points were lower than those of control group significantly (P<0.05 or P<0.01)
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|a CONCLUSION: The downregulation of ACE2 mRNA and protein expression in RPMVECs by endotoxin may play an important role in pathogenesis of ALI
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Endotoxins
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a Peptidyl-Dipeptidase A
|2 NLM
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| 650 |
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7 |
|a EC 3.4.15.1
|2 NLM
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| 650 |
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|a Ace2 protein, rat
|2 NLM
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| 650 |
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7 |
|a EC 3.4.17.23
|2 NLM
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| 650 |
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|a Angiotensin-Converting Enzyme 2
|2 NLM
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| 650 |
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|a EC 3.4.17.23
|2 NLM
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| 700 |
1 |
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|a Zhang, Hong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Geng-yun
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 20(2008), 10 vom: 17. Okt., Seite 582-4
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:20
|g year:2008
|g number:10
|g day:17
|g month:10
|g pages:582-4
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 20
|j 2008
|e 10
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|h 582-4
|