Effect of endotoxin on expression of angiotensin converting enzyme-2 in cultured rat pulmonary microvascular endothelial cells
OBJECTIVE: To observe the effects of endotoxin on angiotensin converting enzyme-2 (ACE2) expression in rat pulmonary microvascular endothelial cells (RPMVECs) in vitro, and discuss the role of ACE2 in endotoxin-induced acute lung injury (ALI)
| Veröffentlicht in: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue. - 1998. - 20(2008), 10 vom: 17. Okt., Seite 582-4 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | Chinese |
| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Endotoxins RNA, Messenger Peptidyl-Dipeptidase A EC 3.4.15.1 Ace2 protein, rat EC 3.4.17.23 Angiotensin-Converting Enzyme 2 |
| Zusammenfassung: | OBJECTIVE: To observe the effects of endotoxin on angiotensin converting enzyme-2 (ACE2) expression in rat pulmonary microvascular endothelial cells (RPMVECs) in vitro, and discuss the role of ACE2 in endotoxin-induced acute lung injury (ALI) METHODS: In vitro, cultured RPMVECs were incubated with endotoxin (10 mg/L), the expression of ACE2 mRNA and protein were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting techniques at time points of 3, 6, 12, and 24 hours respectively after incubation with endotoxin RESULTS: After the treatment of RPMVECs with endotoxin (10 mg/L), the levels of ACE2 mRNA and protein decreased significantly, and then they increased slightly at 6 hours, followed by a continuous decrease until 24 hours. The statistic analysis demonstrated that, except at the 6-hour time point, the levels of ACE2 mRNA and protein expression at other time points were lower than those of control group significantly (P<0.05 or P<0.01) CONCLUSION: The downregulation of ACE2 mRNA and protein expression in RPMVECs by endotoxin may play an important role in pathogenesis of ALI |
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| Beschreibung: | Date Completed 09.03.2010 Date Revised 09.12.2020 published: Print Citation Status MEDLINE |
| ISSN: | 1003-0603 |