In vitro expanded human invariant natural killer T-cells promote functional activity of natural killer cells

In vitro expanded human invariant natural killer T-cells promote functional activity of natural killer cells

Invariant natural killer T (iNKT) cells play a pivotal role in cancer immunity through trans-activation of effector cells via swift cytokine secretion. In mice, iNKT cell activation by alpha-galactosylceramide (alpha-GC) induces potent NK cell-mediated anti-tumour effects. Here we investigated wheth...

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Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 129(2008), 1 vom: 08. Okt., Seite 145-54
1. Verfasser: Moreno, María (VerfasserIn)
Weitere Verfasser: Molling, Johan W, von Mensdorff-Pouilly, Silvia, Verheijen, René H M, von Blomberg, B Mary E, van den Eertwegh, Alfons J M, Scheper, Rik J, Bontkes, Hetty J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Galactosylceramides Interleukins alpha-galactosylceramide Interferon-gamma 82115-62-6 Interleukin-21 MKM3CA6LT1
Beschreibung
Zusammenfassung:Invariant natural killer T (iNKT) cells play a pivotal role in cancer immunity through trans-activation of effector cells via swift cytokine secretion. In mice, iNKT cell activation by alpha-galactosylceramide (alpha-GC) induces potent NK cell-mediated anti-tumour effects. Here we investigated whether human iNKT cells could enhance NK cell functional activity in vitro. iNKT cell activation by alpha-GC treatment of peripheral blood mononuclear cells (PBMC) was not sufficient to enhance NK cell effector functions. However, addition of in vitro expanded iNKT cells to PBMC enhanced NK cell-mediated cytotoxicity in an alpha-GC-dependent manner. NK cell activation by iNKT cells was primarily mediated by soluble factors, and could be enhanced by the NK cell activating cytokine IL-21. These results suggest that adoptive transfer of ex vivo expanded iNKT cells will enhance NK cell function and is expected to enhance the efficacy of cancer immunotherapy, particularly in combination with IL-21 and alpha-GC
Beschreibung:Date Completed 07.10.2008
Date Revised 03.01.2025
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2008.07.004