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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2008.05.006
|2 doi
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|a pubmed24n0602.xml
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|a (DE-627)NLM180609394
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|a (NLM)18595775
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Gu, Ming
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Identification of CTLA-4 isoforms produced by alternative splicing and their association with myasthenia gravis
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| 264 |
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|c 2008
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.09.2008
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|a Date Revised 17.11.2011
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Myasthenia gravis (MG) is an autoimmune disease characterized by muscle weakness induced by autoantibodies against the acetylcholine receptor. CTLA-4 (CD152) plays an inhibitory role in the immune response and has been suggested to be involved in the pathophysiology of MG. In this study, we focused on alternative CTLA-4 mRNA expression in PBMCs from MG patients. We defined two new isoforms of CTLA-4 mRNA that arise due to alternative splicing. By semi-quantitative RT-PCR analysis, we observed a lower expression of sCTLA-4 mRNA relative to the membrane form in MG patients. In addition, the MG patients had lower levels of sCTLA-4 mRNA in PBMCs compared to healthy controls, as assessed by real-time PCR. One of the spliced isoforms (LCTLA-4) was more prevalent in MG patients compared to healthy controls. The alternative splicing was not associated with sex, thymectomy, serum levels of anti-AChR, immunosuppressive treatment or the four CTLA-4 gene polymorphisms analyzed. This study reveals an abnormal spectrum of mRNA expression of CTLA-4 in MG patients, which marks the importance of studying gene expression of alternative splicing
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Antigens, CD
|2 NLM
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| 650 |
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7 |
|a CTLA-4 Antigen
|2 NLM
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| 650 |
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7 |
|a CTLA4 protein, human
|2 NLM
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| 650 |
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7 |
|a Protein Isoforms
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 700 |
1 |
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|a Kakoulidou, Maria
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Giscombe, Ricardo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pirskanen, Ritva
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lefvert, Ann Kari
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Klareskog, Lars
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, XiongBiao
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 128(2008), 3 vom: 15. Sept., Seite 374-81
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
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|g volume:128
|g year:2008
|g number:3
|g day:15
|g month:09
|g pages:374-81
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| 856 |
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|u http://dx.doi.org/10.1016/j.clim.2008.05.006
|3 Volltext
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|h 374-81
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