Isolation and characterization of plasma membrane Na(+)/H(+) antiporter genes from salt-sensitive and salt-tolerant reed plants

Isolation and characterization of plasma membrane Na(+)/H(+) antiporter genes from salt-sensitive and salt-tolerant reed plants

We isolated cDNAs for Na(+)/H(+) antiporter genes (PhaNHA1s) from salt-sensitive and salt-tolerant reed plants. A phylogenetic analysis and localization analysis using yeast strains expressing PhaNHA1-GFP protein showed that PhaNHA1s were plasma membrane Na(+)/H(+) antiporters. Yeast strains express...

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Veröffentlicht in:Journal of plant physiology. - 1979. - 166(2009), 3 vom: 15. Feb., Seite 301-9
1. Verfasser: Takahashi, Ryuichi (VerfasserIn)
Weitere Verfasser: Liu, Shenkui, Takano, Tetsuo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA, Complementary DNA, Plant Recombinant Fusion Proteins Sodium-Hydrogen Exchangers Green Fluorescent Proteins 147336-22-9 Sodium Chloride 451W47IQ8X mehr... Sodium 9NEZ333N27
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245 1 0 |a Isolation and characterization of plasma membrane Na(+)/H(+) antiporter genes from salt-sensitive and salt-tolerant reed plants 
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520 |a We isolated cDNAs for Na(+)/H(+) antiporter genes (PhaNHA1s) from salt-sensitive and salt-tolerant reed plants. A phylogenetic analysis and localization analysis using yeast strains expressing PhaNHA1-GFP protein showed that PhaNHA1s were plasma membrane Na(+)/H(+) antiporters. Yeast strains expressing PhaNHA1 from salt-tolerant reed plants (PhaNHA1-n) grew well than yeast strains expressing PhaNHA1 from salt-sensitive reed plants (PhaNHA1-u) in the presence of 100mM NaCl. Furthermore, Na(+) contents of yeast cells expressing PhaNHA1-n were less than half of those of yeast cells expressing PhaNHA1-u. These results suggest that PhaNHA1-n is more efficient at excluding Na(+) from the cells than PhaNHA1-u 
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700 1 |a Takano, Tetsuo  |e verfasserin  |4 aut 
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