Isolation and characterization of plasma membrane Na(+)/H(+) antiporter genes from salt-sensitive and salt-tolerant reed plants
We isolated cDNAs for Na(+)/H(+) antiporter genes (PhaNHA1s) from salt-sensitive and salt-tolerant reed plants. A phylogenetic analysis and localization analysis using yeast strains expressing PhaNHA1-GFP protein showed that PhaNHA1s were plasma membrane Na(+)/H(+) antiporters. Yeast strains express...
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 166(2009), 3 vom: 15. Feb., Seite 301-9 |
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| Weitere Verfasser: | , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2009
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't DNA, Complementary DNA, Plant Recombinant Fusion Proteins Sodium-Hydrogen Exchangers Green Fluorescent Proteins 147336-22-9 Sodium Chloride 451W47IQ8X mehr... |
| Zusammenfassung: | We isolated cDNAs for Na(+)/H(+) antiporter genes (PhaNHA1s) from salt-sensitive and salt-tolerant reed plants. A phylogenetic analysis and localization analysis using yeast strains expressing PhaNHA1-GFP protein showed that PhaNHA1s were plasma membrane Na(+)/H(+) antiporters. Yeast strains expressing PhaNHA1 from salt-tolerant reed plants (PhaNHA1-n) grew well than yeast strains expressing PhaNHA1 from salt-sensitive reed plants (PhaNHA1-u) in the presence of 100mM NaCl. Furthermore, Na(+) contents of yeast cells expressing PhaNHA1-n were less than half of those of yeast cells expressing PhaNHA1-u. These results suggest that PhaNHA1-n is more efficient at excluding Na(+) from the cells than PhaNHA1-u |
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| Beschreibung: | Date Completed 10.06.2009 Date Revised 30.09.2020 published: Print-Electronic GENBANK: AB244216, AB244218 Citation Status MEDLINE |
| ISSN: | 1618-1328 |
| DOI: | 10.1016/j.jplph.2008.04.002 |