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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2008.04.019
|2 doi
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|a pubmed24n0600.xml
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|e rakwb
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|a eng
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| 100 |
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|a Ma, Weimin
|e verfasserin
|4 aut
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|a Effect of exogenous glucose on the expression and activity of NADPH dehydrogenase complexes in the cyanobacterium Synechocystis sp. strain PCC 6803
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 06.10.2008
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Active NADPH dehydrogenase super- and medium-complexes were newly identified in cyanobacteria and are essential to cyclic photosystem I (PSI) activity and respiration and to CO(2) uptake, respectively. Synechocystis sp. strain PCC 6803 cells were treated with exogenous glucose (Glc) for different times. Active staining of NADPH-nitroblue tetrazolium oxidoreductase and western blot were conducted, and the initial rate of P700(+) dark reduction was measured. The expression and enzyme activity of the NADPH dehydrogenase super-complex were gradually inhibited and were found to be closely associated with the decrease in cyclic PSI activity, as reflected by the initial rate of P700(+) dark reduction. By contrast, those of the NADPH dehydrogenase medium-complex and the activity of CO(2) uptake reflected by the expression levels of NdhD3 and NdhF3 were not significantly affected by the addition of exogenous Glc to the cultures; however, the expression and enzyme activity of this medium-complex were found to be significantly influenced by the changes in CO(2) concentration. These results indicated that (1) the responses of the 2 cyanobacterial NADPH dehydrogenase complexes to exogenous Glc in terms of their expression and activity differed and that (2) these responses were closely associated with their respective physiological roles
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Bacterial Proteins
|2 NLM
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|a Photosynthetic Reaction Center Complex Proteins
|2 NLM
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|a Photosystem I Protein Complex
|2 NLM
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|a Carbon Dioxide
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|a 142M471B3J
|2 NLM
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|a NADPH Dehydrogenase
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|a Glucose
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|4 aut
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 46(2008), 8-9 vom: 15. Aug., Seite 775-9
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|g volume:46
|g year:2008
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|g day:15
|g month:08
|g pages:775-9
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|u http://dx.doi.org/10.1016/j.plaphy.2008.04.019
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