Effect of exogenous glucose on the expression and activity of NADPH dehydrogenase complexes in the cyanobacterium Synechocystis sp. strain PCC 6803
Active NADPH dehydrogenase super- and medium-complexes were newly identified in cyanobacteria and are essential to cyclic photosystem I (PSI) activity and respiration and to CO(2) uptake, respectively. Synechocystis sp. strain PCC 6803 cells were treated with exogenous glucose (Glc) for different ti...
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 46(2008), 8-9 vom: 15. Aug., Seite 775-9 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Bacterial Proteins Photosynthetic Reaction Center Complex Proteins Photosystem I Protein Complex Carbon Dioxide 142M471B3J NADPH Dehydrogenase EC 1.6.99.1 Glucose |
| Zusammenfassung: | Active NADPH dehydrogenase super- and medium-complexes were newly identified in cyanobacteria and are essential to cyclic photosystem I (PSI) activity and respiration and to CO(2) uptake, respectively. Synechocystis sp. strain PCC 6803 cells were treated with exogenous glucose (Glc) for different times. Active staining of NADPH-nitroblue tetrazolium oxidoreductase and western blot were conducted, and the initial rate of P700(+) dark reduction was measured. The expression and enzyme activity of the NADPH dehydrogenase super-complex were gradually inhibited and were found to be closely associated with the decrease in cyclic PSI activity, as reflected by the initial rate of P700(+) dark reduction. By contrast, those of the NADPH dehydrogenase medium-complex and the activity of CO(2) uptake reflected by the expression levels of NdhD3 and NdhF3 were not significantly affected by the addition of exogenous Glc to the cultures; however, the expression and enzyme activity of this medium-complex were found to be significantly influenced by the changes in CO(2) concentration. These results indicated that (1) the responses of the 2 cyanobacterial NADPH dehydrogenase complexes to exogenous Glc in terms of their expression and activity differed and that (2) these responses were closely associated with their respective physiological roles |
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| Beschreibung: | Date Completed 06.10.2008 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2008.04.019 |