Clonal CD8+ TCR-Vbeta expanded populations with effector memory phenotype in Churg Strauss syndrome

Clonal CD8+ TCR-Vbeta expanded populations with effector memory phenotype in Churg Strauss syndrome

Churg Strauss Syndrome (CSS) is a systemic vasculitis in which oligoclonal T cell expansions might be involved in the pathogenesis. Combined analysis of TCR-Vbeta expression profile by flow cytometry and of TCR gene rearrangement by heteroduplex PCR was used to detect and characterize T cell expansi...

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Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 128(2008), 1 vom: 01. Juli, Seite 94-102
1. Verfasser: Guida, Giuseppe (VerfasserIn)
Weitere Verfasser: Vallario, Antonella, Stella, Stefania, Boita, Monica, Circosta, Paola, Mariani, Sara, Prato, Giuseppina, Heffler, Enrico, Bergia, Roberta, Sottile, Antonino, Rolla, Giovanni, Cignetti, Alessandro
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Biomarkers Receptors, Antigen, T-Cell, alpha-beta
Beschreibung
Zusammenfassung:Churg Strauss Syndrome (CSS) is a systemic vasculitis in which oligoclonal T cell expansions might be involved in the pathogenesis. Combined analysis of TCR-Vbeta expression profile by flow cytometry and of TCR gene rearrangement by heteroduplex PCR was used to detect and characterize T cell expansions in 8 CSS patients, 10 asthmatics and 42 healthy subjects. In all CSS patients one or two Vbeta families were expanded among CD8+ cells, with an effector memory phenotype apt to populate tissues and inflammatory sites. Heteroduplex PCR showed the presence of one or more clonal TCR rearrangements, which reveals monoclonal or oligoclonal T cells subpopulations. After purification with a Vbeta specific monoclonal antibody, each CD8+/Vbeta+ expanded family showed a single TCR rearrangement, clearly suggestive of monoclonality. All CD8+ expansions were detectable throughout the disease course. TCR-Vbeta expanded or deleted populations were not observed in asthmatic patients. Clonal CD8+/Vbeta+ T cell expansions might be useful as a disease marker
Beschreibung:Date Completed 15.07.2008
Date Revised 19.11.2015
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2008.03.505