RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe
Here we describe a method that allows selective detection of silent copy transcripts in homothallic strains of Schizosaccharomyces pombe in the presence of the active cassettes. The method involving RT-PCR (reverse transcriptase polymerase chain reaction) exploits our observation that the silent cop...
| Publié dans: | BioTechniques. - 1991. - 44(2008), 1 vom: Jan., Seite 54, 56, 58 |
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| Auteur principal: | |
| Autres auteurs: | |
| Format: | Article |
| Langue: | English |
| Publié: |
2008
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Chromosomal Proteins, Non-Histone RNA, Fungal RNA, Messenger Schizosaccharomyces pombe Proteins Swi6 protein, S pombe |
| Résumé: | Here we describe a method that allows selective detection of silent copy transcripts in homothallic strains of Schizosaccharomyces pombe in the presence of the active cassettes. The method involving RT-PCR (reverse transcriptase polymerase chain reaction) exploits our observation that the silent copy transcripts extend beyond the regions of homology to the flanking sequences specific for the donor cassettes, thus allowing design of oligos that are specific for the different donors. The results are validated using a known silencing mutant swi6 |
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| Description: | Date Completed 25.02.2008 Date Revised 07.06.2018 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |