RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe
Here we describe a method that allows selective detection of silent copy transcripts in homothallic strains of Schizosaccharomyces pombe in the presence of the active cassettes. The method involving RT-PCR (reverse transcriptase polymerase chain reaction) exploits our observation that the silent cop...
| Publié dans: | BioTechniques. - 1991. - 44(2008), 1 vom: Jan., Seite 54, 56, 58 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | |
| Format: | Article |
| Langue: | English |
| Publié: |
2008
|
| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Chromosomal Proteins, Non-Histone RNA, Fungal RNA, Messenger Schizosaccharomyces pombe Proteins Swi6 protein, S pombe |