Dynamics of membrane adhesion : the role of polyethylene glycol spacers, ligand-receptor bond strength, and rupture pathway
Biological adhesion typically occurs through discrete cross bridges between complementary molecules on adjacent membranes. Here we report quantitative measurements of the binding distance between a lipid membrane functionalized with ligands on flexible polymer tether chains and a second membrane bea...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 24(2008), 4 vom: 19. Feb., Seite 1225-31 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Ligands Lipid Bilayers Phosphatidylethanolamines 1,2-distearoylphosphatidylethanolamine 1G4B5265CQ Polyethylene Glycols 3WJQ0SDW1A mehr... |
| Zusammenfassung: | Biological adhesion typically occurs through discrete cross bridges between complementary molecules on adjacent membranes. Here we report quantitative measurements of the binding distance between a lipid membrane functionalized with ligands on flexible polymer tether chains and a second membrane bearing complementary receptors using the surface force apparatus technique. The binding distance is shown to increase as a function of polymer tether length. Upon separation, adhesive failure occurs not at the strong ligand-receptor bond but primarily through the mechanical pullout of cross-bridging polymer tethers from the membrane. We summarize these measurements of complementary membrane adhesion dynamics using an energy-state diagram that encompasses the energetics of the polymer tether, ligand-receptor bond strength, and number of cross bridges formed |
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| Beschreibung: | Date Completed 07.05.2008 Date Revised 01.12.2018 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la702357a |