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231223s2007 xx ||||| 00| ||eng c |
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Yuan, Weiyong
|e verfasserin
|4 aut
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| 245 |
1 |
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|a pH-controlled construction of chitosan/alginate multilayer film
|b characterization and application for antibody immobilization
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| 264 |
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|c 2007
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 28.01.2008
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|a Date Revised 01.12.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a In this work, a chitosan/alginate multilayer film was constructed via layer-by-layer self-assembly and studied by in situ surface plasmon resonance monitoring and contact angle measurements. The results demonstrate that the surface composition of the self-assembled multilayer film can be simply tailored through pH control during the assembly process. The biological property of the assembled film was further characterized via antigen-antibody interactions, showing that the loading capacity of the antibody on the multilayered film and the binding activity of the antigen to the immobilized antibody could be also well-tuned by pH control. This work can provide more scientific insight in the interaction between protein and polymer matrix and render a novel simple approach to build high-performance biointerfaces through pH control for potential applications of highly sensitive immunosensors
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Alginates
|2 NLM
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|a Antibodies
|2 NLM
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|a Antigen-Antibody Complex
|2 NLM
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| 650 |
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7 |
|a Hexuronic Acids
|2 NLM
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| 650 |
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7 |
|a Glucuronic Acid
|2 NLM
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| 650 |
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|a 8A5D83Q4RW
|2 NLM
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| 650 |
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|a Chitosan
|2 NLM
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| 650 |
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|a 9012-76-4
|2 NLM
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| 700 |
1 |
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|a Dong, Hua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Chang Ming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cui, Xiaoqiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Ling
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Zhisong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhou, Qin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1991
|g 23(2007), 26 vom: 18. Dez., Seite 13046-52
|w (DE-627)NLM098181009
|x 0743-7463
|7 nnns
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| 773 |
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|g volume:23
|g year:2007
|g number:26
|g day:18
|g month:12
|g pages:13046-52
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