pH-controlled construction of chitosan/alginate multilayer film : characterization and application for antibody immobilization
In this work, a chitosan/alginate multilayer film was constructed via layer-by-layer self-assembly and studied by in situ surface plasmon resonance monitoring and contact angle measurements. The results demonstrate that the surface composition of the self-assembled multilayer film can be simply tail...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1991. - 23(2007), 26 vom: 18. Dez., Seite 13046-52 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2007
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Alginates Antibodies Antigen-Antibody Complex Hexuronic Acids Glucuronic Acid 8A5D83Q4RW Chitosan 9012-76-4 |
| Zusammenfassung: | In this work, a chitosan/alginate multilayer film was constructed via layer-by-layer self-assembly and studied by in situ surface plasmon resonance monitoring and contact angle measurements. The results demonstrate that the surface composition of the self-assembled multilayer film can be simply tailored through pH control during the assembly process. The biological property of the assembled film was further characterized via antigen-antibody interactions, showing that the loading capacity of the antibody on the multilayered film and the binding activity of the antigen to the immobilized antibody could be also well-tuned by pH control. This work can provide more scientific insight in the interaction between protein and polymer matrix and render a novel simple approach to build high-performance biointerfaces through pH control for potential applications of highly sensitive immunosensors |
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| Beschreibung: | Date Completed 28.01.2008 Date Revised 01.12.2018 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 0743-7463 |