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231223s2007 xx ||||| 00| ||eng c |
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|a (NLM)17929953
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Chow, Dominic C
|e verfasserin
|4 aut
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| 245 |
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|a Surface-initiated enzymatic polymerization of DNA
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 15.01.2008
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|a Date Revised 24.11.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We describe a technique to synthesize DNA homopolymers on a surface using surface-initiated enzymatic polymerization (SIEP) with terminal deoxynucleotidyl transferase (TdTase), an enzyme that repetitively adds mononucleotides to the 3'-end of oligonucleotides. The thickness of the synthesized DNA layer was found to depend on the deoxymononucleotide monomer, in the order of dATP > dTTP > dGTP approximately dCTP. In addition, the composition and the surface density of oligonucleotide initiators were also important in controlling the extent of DNA polymerization. The extension of single-stranded DNA chains by SIEP was further verified by their binding to antibodies specific to oligonucleotides. TdTase-mediated SIEP can also be used to grow spatially defined three-dimensional DNA structures by soft lithography and is a new tool for bioinspired fabrication at the micro- and nanoscale
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|a Comparative Study
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Antibodies
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|a DNA, Single-Stranded
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|a Deoxyadenine Nucleotides
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|a Deoxyguanine Nucleotides
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|a Oligonucleotides
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|a Polydeoxyribonucleotides
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|a Thymine Nucleotides
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|a deoxyguanosine triphosphate
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|a DNA Nucleotidylexotransferase
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|a EC 2.7.7.31
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| 650 |
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|a 2'-deoxyadenosine triphosphate
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|a thymidine 5'-triphosphate
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|a Chilkoti, Ashutosh
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 23(2007), 23 vom: 06. Nov., Seite 11712-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:23
|g year:2007
|g number:23
|g day:06
|g month:11
|g pages:11712-7
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