Surface-initiated enzymatic polymerization of DNA

We describe a technique to synthesize DNA homopolymers on a surface using surface-initiated enzymatic polymerization (SIEP) with terminal deoxynucleotidyl transferase (TdTase), an enzyme that repetitively adds mononucleotides to the 3'-end of oligonucleotides. The thickness of the synthesized D...

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Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 23(2007), 23 vom: 06. Nov., Seite 11712-7
1. Verfasser: Chow, Dominic C (VerfasserIn)
Weitere Verfasser: Chilkoti, Ashutosh
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Antibodies DNA, Single-Stranded Deoxyadenine Nucleotides Deoxyguanine Nucleotides Oligonucleotides Polydeoxyribonucleotides Thymine Nucleotides mehr... deoxyguanosine triphosphate 8C2O37Y44Q DNA Nucleotidylexotransferase EC 2.7.7.31 2'-deoxyadenosine triphosphate K8KCC8SH6N thymidine 5'-triphosphate QOP4K539MU