Intercalation interactions between dsDNA and acridine studied by single molecule force spectroscopy
In this letter, we report on the direct measurement of the intercalation interactions between acridine and double-stranded DNA (dsDNA) using single molecule force spectroscopy. The interaction between acridine and dsDNA is broken by force of 36 pN at a loading rate of 5.0 nN/s. The most probable rup...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 23(2007), 18 vom: 28. Aug., Seite 9140-2 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2007
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| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Acridines Intercalating Agents DNA 9007-49-2 |
| Résumé: | In this letter, we report on the direct measurement of the intercalation interactions between acridine and double-stranded DNA (dsDNA) using single molecule force spectroscopy. The interaction between acridine and dsDNA is broken by force of 36 pN at a loading rate of 5.0 nN/s. The most probable rupture force between acridine and dsDNA is dependent on the loading rate, indicating that the binding of acridine and dsDNA is a dynamic process. The combination of SMFS experimental data with the theoretical model clearly suggests the presence of two energy barriers along with an unbinding trajectory of acridine-dsDNA |
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| Description: | Date Completed 02.10.2007 Date Revised 21.08.2007 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |