Sensitivity enhancement in saturation transfer difference (STD) experiments through optimized excitation schemes
Investigation of ligand-protein interactions by the saturation transfer difference (STD) experiment has been well established in the drug discovery process through numerous examples. Thus, binding epitopes may be mapped by comparing signals of the ligand with and without saturation of the protein. H...
| Veröffentlicht in: | Magnetic resonance in chemistry : MRC. - 1985. - 45(2007), 9 vom: 06. Sept., Seite 720-4 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2007
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| Zugriff auf das übergeordnete Werk: | Magnetic resonance in chemistry : MRC |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't E-Selectin Epitopes Glycoproteins Ligands Myelin-Associated Glycoprotein Serum Albumin |
| Zusammenfassung: | Investigation of ligand-protein interactions by the saturation transfer difference (STD) experiment has been well established in the drug discovery process through numerous examples. Thus, binding epitopes may be mapped by comparing signals of the ligand with and without saturation of the protein. Herein, it is shown that a less selective process allows more protons to assist in the saturation of the protein, thereby considerably enhancing the sensitivity of the STD experiment. Increasing the saturation power entails a greater risk of perturbing the ligand; however, an amplitude modulation of the waveform assists this procedure by distributing the applied energy in sidebands |
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| Beschreibung: | Date Completed 12.10.2007 Date Revised 06.08.2007 published: Print Citation Status MEDLINE |
| ISSN: | 1097-458X |